#200 steps of minimization, distance-dependent dielectric in AMBER7
 
    maxcyc=200, imin=1, ntmin=1, ncyc=100, nsnb=20,
    igb=0,ntb=0,cut=10.0, dielc=1.0, ntpr=10,
    ntr=1, 
 
 
    eedmeth=5,
 
restrain protein atoms except H, ligand, and wat
32.0
FIND
* CT * *
* C * *
* C* * *
SEARCH
RES 1 388
END
restrain protein atoms except H
32.0
FIND 
* CA * *
* CB * *
* CC * *
* CK * *
* CM * *
* CN * *
* CQ * *
* CR * *
* CV * *
* CW * *
SEARCH
RES 1 388
END
restrain protein atoms except H
32.0
FIND
* N3 * *
* N * *
* N* * *
* N2 * *
* NA * *
* NB * *
* NC * *
SEARCH
RES 1 388
END
restrain protein atoms except H
32.0
FIND
* OH * *
* OS * *
* O * *
* O2 * *
* P * *
* S * *
* SH * *
SEARCH
RES 1 388
END 
END
 
 
